In this video Deimante is performing a luciferase assay! Luciferase is an enzyme that’s responsible for facilitating bioluminescence, the production of light from living organisms. This assay measures how much luciferase is present in a sample. In her research of viral entry using pseudoviruses (engineered/fake viruses), she is able to see whether the pseudovirus has entered cells. Deimante is currently an MRes student studying the entry of human coronaviruses, looking for unknown receptors and/
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A luciferase assay is a bioluminescent technique used to measure gene expression, promoter strength, or protein activity. It works by using the luciferase enzyme to catalyze the oxidation of a substrate called luciferin, which produces light. The intensity of the light emitted is directly proportional to the amount of luciferase enzyme, which in turn reflects the activity of the gene or promoter being studied. | Tsouryel Ben Israel
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